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Flow Cytometry · Haematopathology Reference

CD5-Based Immunophenotypes
in B-Cell LPDs

Interactive diagnostic navigator for mature B-cell lymphoproliferative disorders — structured around CD5 and CD10 expression as primary branching criteria.

⚠ Educational tool only. Based on classic immunophenotypic patterns from haematopathology references. Not intended for clinical diagnosis. Always integrate morphology, genetics, clinical context, and consult a haematopathologist for case-specific interpretation.
Entry — Step 01
Is the B-cell population CD5 positive?
Most common branching point in flow cytometric analysis of mature B-cell LPDs. Aberrant CD5 expression on B-cells is the key discriminator between two major diagnostic streams.

Clinical notes

  • DLBCL can emerge in any branch — CD5+ subtype exists and carries worse prognosis.
  • Always correlate with morphology and additional IHC: Cyclin D1, SOX11, LEF1, BCL6, MYC.
  • MYD88 L265P is present in ~90–95% of LPL but rare in MZL — a critical discriminator.
  • Some DLBCL cases require gene expression profiling (GCB vs. ABC subtyping) for definitive classification.
  • HCL-variant and splenic MZL can share features; BRAF V600E strongly favours classic HCL.
CD5-positive B-cell LPDs
CLL / SLL
CD5+ CD23+ CD200 bright CD20 dim sIg dim Cyclin D1− LEF1+
Mantle Cell Lymphoma
CD5+ CD23− CD200−/dim Cyclin D1+ t(11;14) SOX11+ CD20 bright
CD5-negative, CD10-positive B-cell LPDs
Follicular Lymphoma
CD5− CD10+ BCL2+ BCL6+ t(14;18)
Burkitt Lymphoma
CD5− CD10+ BCL2− MYC rearranged Ki-67 ~100% TdT−
DLBCL (GCB type)
CD5−/+ CD10+/− BCL6+ GEP subtyping MYC/BCL2 DHL
CD5-negative, CD10-negative B-cell LPDs
Hairy Cell Leukemia
CD5− CD10− CD103+ CD25+ CD11c bright CD123+ BRAF V600E
Lymphoplasmacytic Lymphoma
CD5− CD10− cytoplasmic Ig+ CD38+ MYD88 L265P ~95%
Marginal Zone Lymphoma
CD5− CD10− BCL6− MYD88 wild-type Site-dependent subtypes